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human neonatal foreskin fibroblasts bj  (ATCC)


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    ATCC human neonatal foreskin fibroblasts bj
    Human Neonatal Foreskin Fibroblasts Bj, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1788 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+neonatal+foreskin+bj+fibroblasts/BJ/pm40722928-74-24-32
    Average 99 stars, based on 1788 article reviews
    human neonatal foreskin fibroblasts bj - by Bioz Stars, 2026-09
    99/100 stars

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    Derivative Assay:

    Article Title: Induced endothelial cells from peripheral arterial disease patients and neonatal fibroblasts have comparable angiogenic properties
    Article Snippet: .. Patient derived adult fibroblasts, and human neonatal foreskin BJ fibroblasts (American Type Culture Collection (ATCC), Manassas, VA 20108), were seeded on 6-well gelatine coated plates in DMEM containing 10% FBS (with 1% NaPyruvate, penicillin and streptomycin, L-glutamine) overnight before transfection. .. Cells were transfected over 14 days, with mmRNA (RNACore, 6670 Bertner Avenue, Houston, Texas 77030) encoding ETV2, FLI1, GATA2, and KLF4 each day in the presence of Lipofectamine RNAiMAX (13778–075, L3484, Life Technologies Australia Pty Ltd., Scoresby, VIC 3179).

    Transfection:

    Article Title: Induced endothelial cells from peripheral arterial disease patients and neonatal fibroblasts have comparable angiogenic properties
    Article Snippet: .. Patient derived adult fibroblasts, and human neonatal foreskin BJ fibroblasts (American Type Culture Collection (ATCC), Manassas, VA 20108), were seeded on 6-well gelatine coated plates in DMEM containing 10% FBS (with 1% NaPyruvate, penicillin and streptomycin, L-glutamine) overnight before transfection. .. Cells were transfected over 14 days, with mmRNA (RNACore, 6670 Bertner Avenue, Houston, Texas 77030) encoding ETV2, FLI1, GATA2, and KLF4 each day in the presence of Lipofectamine RNAiMAX (13778–075, L3484, Life Technologies Australia Pty Ltd., Scoresby, VIC 3179).



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    human neonatal foreskin dermal fibroblasts  (ATCC)
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    Figure 1: The protein expression and activity of TG2 in young and chronologically aged human dermal fibroblasts. (A) TG2 amounts were determined in young (passage no 4 and 10) and aged fibroblasts (passage no 26 and 32) by Western blot analysis using monoclonal anti TG2 antibody (1:2,000) and anti-GAPDH antibody (1:2,000). (B) The enzymatic activity of TG2 in the cells from passages 10 and 26 was determined by in situ TG2 activity assay using HRP-streptavidin antibody (1:2,000) and BPA incorporated cellular proteins were visualized. Similar results were ob- tained in three independent experiments.
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    bj human neonatal foreskin fibroblasts  (ATCC)
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    Figure 1: The protein expression and activity of TG2 in young and chronologically aged human dermal fibroblasts. (A) TG2 amounts were determined in young (passage no 4 and 10) and aged fibroblasts (passage no 26 and 32) by Western blot analysis using monoclonal anti TG2 antibody (1:2,000) and anti-GAPDH antibody (1:2,000). (B) The enzymatic activity of TG2 in the cells from passages 10 and 26 was determined by in situ TG2 activity assay using HRP-streptavidin antibody (1:2,000) and BPA incorporated cellular proteins were visualized. Similar results were ob- tained in three independent experiments.
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    Figure 1: The protein expression and activity of TG2 in young and chronologically aged human dermal fibroblasts. (A) TG2 amounts were determined in young (passage no 4 and 10) and aged fibroblasts (passage no 26 and 32) by Western blot analysis using monoclonal anti TG2 antibody (1:2,000) and anti-GAPDH antibody (1:2,000). (B) The enzymatic activity of TG2 in the cells from passages 10 and 26 was determined by in situ TG2 activity assay using HRP-streptavidin antibody (1:2,000) and BPA incorporated cellular proteins were visualized. Similar results were ob- tained in three independent experiments.
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    ATCC human neonatal foreskin bj fibroblasts
    Figure 1: The protein expression and activity of TG2 in young and chronologically aged human dermal fibroblasts. (A) TG2 amounts were determined in young (passage no 4 and 10) and aged fibroblasts (passage no 26 and 32) by Western blot analysis using monoclonal anti TG2 antibody (1:2,000) and anti-GAPDH antibody (1:2,000). (B) The enzymatic activity of TG2 in the cells from passages 10 and 26 was determined by in situ TG2 activity assay using HRP-streptavidin antibody (1:2,000) and BPA incorporated cellular proteins were visualized. Similar results were ob- tained in three independent experiments.
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    neonatal human foreskin fibroblast bj  (ATCC)
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    ATCC neonatal human foreskin fibroblast bj
    Figure 1: The protein expression and activity of TG2 in young and chronologically aged human dermal fibroblasts. (A) TG2 amounts were determined in young (passage no 4 and 10) and aged fibroblasts (passage no 26 and 32) by Western blot analysis using monoclonal anti TG2 antibody (1:2,000) and anti-GAPDH antibody (1:2,000). (B) The enzymatic activity of TG2 in the cells from passages 10 and 26 was determined by in situ TG2 activity assay using HRP-streptavidin antibody (1:2,000) and BPA incorporated cellular proteins were visualized. Similar results were ob- tained in three independent experiments.
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    neonatal foreskin human cells  (ATCC)
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    Figure 1: The protein expression and activity of TG2 in young and chronologically aged human dermal fibroblasts. (A) TG2 amounts were determined in young (passage no 4 and 10) and aged fibroblasts (passage no 26 and 32) by Western blot analysis using monoclonal anti TG2 antibody (1:2,000) and anti-GAPDH antibody (1:2,000). (B) The enzymatic activity of TG2 in the cells from passages 10 and 26 was determined by in situ TG2 activity assay using HRP-streptavidin antibody (1:2,000) and BPA incorporated cellular proteins were visualized. Similar results were ob- tained in three independent experiments.
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    Image Search Results


    Figure 1: The protein expression and activity of TG2 in young and chronologically aged human dermal fibroblasts. (A) TG2 amounts were determined in young (passage no 4 and 10) and aged fibroblasts (passage no 26 and 32) by Western blot analysis using monoclonal anti TG2 antibody (1:2,000) and anti-GAPDH antibody (1:2,000). (B) The enzymatic activity of TG2 in the cells from passages 10 and 26 was determined by in situ TG2 activity assay using HRP-streptavidin antibody (1:2,000) and BPA incorporated cellular proteins were visualized. Similar results were ob- tained in three independent experiments.

    Journal: Turkish Journal of Biochemistry

    Article Title: Identification of the role of TG2 on the expression of TGF-β, TIMP-1 and TIMP-2 in aged skin

    doi: 10.1515/tjb-2023-0235

    Figure Lengend Snippet: Figure 1: The protein expression and activity of TG2 in young and chronologically aged human dermal fibroblasts. (A) TG2 amounts were determined in young (passage no 4 and 10) and aged fibroblasts (passage no 26 and 32) by Western blot analysis using monoclonal anti TG2 antibody (1:2,000) and anti-GAPDH antibody (1:2,000). (B) The enzymatic activity of TG2 in the cells from passages 10 and 26 was determined by in situ TG2 activity assay using HRP-streptavidin antibody (1:2,000) and BPA incorporated cellular proteins were visualized. Similar results were ob- tained in three independent experiments.

    Article Snippet: Human neonatal foreskin (dermal) fibroblasts (BJ ATCC® CRL-2522TM) were cultured in DMEM (ThermoFisher, 41965039) medium supplemented with 10 % fetal bovine serum (FBS) (Thermo Fisher, 10270106), and penicillin/streptomycin antibiotics (ThermoFisher, 15140122) at 37 °C in an atmosphere of 5 % CO2.

    Techniques: Expressing, Activity Assay, Western Blot, In Situ

    Figure 2: The protein expression of TGF-β, TIMP-1 and TIMP-2 in ZDON treated human dermal fibroblasts. (A) Chronologically aged human dermal fibroblasts (passage no 26) were treated with TG2 active site inhibitor ZDON (50 μM) for 21 h and in situ TG2 activity assay was performed as described in materials and methods. Untreated cells were used as control (CTR). BPA incorporated cellular proteins (TG2 activity) were visualized (TG2 ACT: TG2 activity). (B) The protein levels of TGF-β, TIMP-1 and TIMP-2 were determined in ZDON treated and untreated (CTR) cells using anti-TGF-β antibody (10 μg/mL), anti-TIMP-1 antibody (4 μg/mL), anti-TIMP-2 antibody (1:1,000) and anti-GAPDH antibody (1:2,000). (C) Cell viability of ZDON treated and untreated human dermal fibroblasts were also visualized. All experiments were repeated at least three times with the cells from passage no 26 and 32. Similar results were obtained. Data are represented as means ± SEM, *p<0.05, ns, not significant.

    Journal: Turkish Journal of Biochemistry

    Article Title: Identification of the role of TG2 on the expression of TGF-β, TIMP-1 and TIMP-2 in aged skin

    doi: 10.1515/tjb-2023-0235

    Figure Lengend Snippet: Figure 2: The protein expression of TGF-β, TIMP-1 and TIMP-2 in ZDON treated human dermal fibroblasts. (A) Chronologically aged human dermal fibroblasts (passage no 26) were treated with TG2 active site inhibitor ZDON (50 μM) for 21 h and in situ TG2 activity assay was performed as described in materials and methods. Untreated cells were used as control (CTR). BPA incorporated cellular proteins (TG2 activity) were visualized (TG2 ACT: TG2 activity). (B) The protein levels of TGF-β, TIMP-1 and TIMP-2 were determined in ZDON treated and untreated (CTR) cells using anti-TGF-β antibody (10 μg/mL), anti-TIMP-1 antibody (4 μg/mL), anti-TIMP-2 antibody (1:1,000) and anti-GAPDH antibody (1:2,000). (C) Cell viability of ZDON treated and untreated human dermal fibroblasts were also visualized. All experiments were repeated at least three times with the cells from passage no 26 and 32. Similar results were obtained. Data are represented as means ± SEM, *p<0.05, ns, not significant.

    Article Snippet: Human neonatal foreskin (dermal) fibroblasts (BJ ATCC® CRL-2522TM) were cultured in DMEM (ThermoFisher, 41965039) medium supplemented with 10 % fetal bovine serum (FBS) (Thermo Fisher, 10270106), and penicillin/streptomycin antibiotics (ThermoFisher, 15140122) at 37 °C in an atmosphere of 5 % CO2.

    Techniques: Expressing, In Situ, Activity Assay, Control

    Figure 3: The protein expression of TGF-β, TIMP-1 and TIMP-2 in TG2 downregulated human dermal fibroblasts. (A) Chronologically aged human dermal fibroblasts (passage no 26) were transfected with TG2 specific siRNA (50 nM) as described in materials and methods and scRNA transfected cells were used as control (CTR). TG2 and GAPDH antibodies were used in 1:2,000 ratio. Two lanes for each siRNA and CTR represent TG2 bands in parallel loadings from the same lysate. (B) The protein levels of TGF-β, TIMP-1 and TIMP-2 were determined in siRNA transfected (TG2 downregulated) cells and compared with scRNA transfected control cells (CTR) using anti-TGF-β antibody (10 μg/mL), anti-TIMP-1 antibody (4 μg/mL), anti-TIMP-2 antibody (1:1,000) and anti-GAPDH antibody (1:2,000). All experiments were repeated at least three times with the cells from passage no 26 and 32. Similar results were obtained. Data are represented as means ± SEM, *p<0.05, ns, not significant.

    Journal: Turkish Journal of Biochemistry

    Article Title: Identification of the role of TG2 on the expression of TGF-β, TIMP-1 and TIMP-2 in aged skin

    doi: 10.1515/tjb-2023-0235

    Figure Lengend Snippet: Figure 3: The protein expression of TGF-β, TIMP-1 and TIMP-2 in TG2 downregulated human dermal fibroblasts. (A) Chronologically aged human dermal fibroblasts (passage no 26) were transfected with TG2 specific siRNA (50 nM) as described in materials and methods and scRNA transfected cells were used as control (CTR). TG2 and GAPDH antibodies were used in 1:2,000 ratio. Two lanes for each siRNA and CTR represent TG2 bands in parallel loadings from the same lysate. (B) The protein levels of TGF-β, TIMP-1 and TIMP-2 were determined in siRNA transfected (TG2 downregulated) cells and compared with scRNA transfected control cells (CTR) using anti-TGF-β antibody (10 μg/mL), anti-TIMP-1 antibody (4 μg/mL), anti-TIMP-2 antibody (1:1,000) and anti-GAPDH antibody (1:2,000). All experiments were repeated at least three times with the cells from passage no 26 and 32. Similar results were obtained. Data are represented as means ± SEM, *p<0.05, ns, not significant.

    Article Snippet: Human neonatal foreskin (dermal) fibroblasts (BJ ATCC® CRL-2522TM) were cultured in DMEM (ThermoFisher, 41965039) medium supplemented with 10 % fetal bovine serum (FBS) (Thermo Fisher, 10270106), and penicillin/streptomycin antibiotics (ThermoFisher, 15140122) at 37 °C in an atmosphere of 5 % CO2.

    Techniques: Expressing, Transfection, Control